Journal: Pharmaceutical Biology
Article Title: Diosgenin protects against cationic bovine serum albumin-induced membranous glomerulonephritis by attenuating oxidative stress and renal inflammation via the NF-κB pathway
doi: 10.1080/13880209.2024.2330602
Figure Lengend Snippet: DG inactivates of the NF-κB signalling pathway in MGN rats. (A) Protein levels of NF-κB p65, IKKβ, p-IKKβ, ICAM-1, VCAM-1, MCP-1, E-selectin, nuclear NF-κB p65 and cytosolic NF-κB p65 were evaluated by western blotting. (B–J) Ratio of NF-κB p65, IKKβ, p-IKKβ, ICAM-1, VCAM-1, MCP-1, E-selectin, nuclear NF-κB p65 and cytosolic NF-κB p65. Data are expressed as the mean ± standard deviation (SD), n = 3. # p < 0.05 or ## p < 0.01 vs. NC group. * p < 0.05 or ** p < 0.01 vs. MGN group. NC: normal control; MGN: membranous glomerulonephritis; DG: diosgenin; TPCA1: [(aminocarbony)amino]-5-(4-fluorophenyl)-3-thiophenecarboxamide.
Article Snippet: Anti-rabbit antibodies against nuclear factor-κB p65 (NF-κB p65, cat. no. D14E12, RRID: AB_2799359), IκB kinase-β (IKKβ, cat. no. D30C6) and p-IKKβ (cat. no. 16A6) were purchased from Cell Signaling Technology (Danvers, MA).
Techniques: Western Blot, Standard Deviation